Alkaline Phosphatase

The Effects of varying p-Nitrophenyl Phosphate concentrations on saltlike Phosphatase body dish out Nathan Overholt Louis Settembrino March 5, 2002 Abstract:         The enzyme saltlike Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time seek was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm, and the Km was bring in to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction: Alkaline Phosphatase: Alkaline Phosphatase is an enzyme that comes from liver, off-white, placenta, and intestine. This enzyme hydrolyses phosphate esters, is secreted into the serum by osteoblasts and is used as a diagnostic marker for increased metabolic activity. ontogeny swots need base-forming phosphatase. Any check up on of lift emersion will cause alkaline phosphatase levels to rise. The condition may be normal, much(prenominal) as a childhood growth spurt or the healing of a broken bone; or the condition may be a disease, such as bone cancer, Pagets disease, or rickets. During pregnancy, alkaline phosphatase is made by the placenta and leaks into the mothers bloodstream. This is normal. Some tumors, however, graduation exercise production of the same kind of alkaline phosphatase produced by the placenta. These tumors ar called germ cell tumors and include testicular cancer and trustworthy brain tumors.

(Nordensen, 2002) Healthy livers usually oblige alkaline phosphatase onward along with other harmful substances come on through and through the bile duct. If th! e liver becomes diseased in almost sort, thence the bile duct may become block up producing large amounts of built up alkaline phosphatase and eventually this leaks erupt into the bloodstream. There are different forms of alkaline phosphatase called isoenzymes. The determination of the lark isoenzyme will determine the target problem area. (Sorensen, 2002) Enzymes: Enzymes are catalysts, meaning, they pop off a reaction proceed without being consumed. The substrate binds to a... If you uttermost to get a full essay, order it on our website: OrderCustomPaper.com

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